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murine respirovirus  (ATCC)


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    Structured Review

    ATCC murine respirovirus
    Red indicates passed and black failed. RNA from murine <t>respirovirus,</t> orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.
    Murine Respirovirus, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+respirovirus/Quantitative+Genomic+RNA+from+Sendai+virus+strain+Cantell/med_rxiv__64898__2026__02__06__26345651-182-29-31
    Average 94 stars, based on 2 article reviews
    murine respirovirus - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens"

    Article Title: Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens

    Journal: medRxiv

    doi: 10.64898/2026.02.06.26345651

    Red indicates passed and black failed. RNA from murine respirovirus, orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.
    Figure Legend Snippet: Red indicates passed and black failed. RNA from murine respirovirus, orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.

    Techniques Used: Virus, cDNA Synthesis, Extraction, Control, Biomarker Discovery

    Related Articles

    Virus:

    Article Title: Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens
    Article Snippet: First strand cDNA was synthesized using the Maxima H Minus 1 st Strand cDNA synthesis kit with ds DNase (Thermo Fisher Scientific), in a 20μl reaction volume, according to the manufacturer’s protocol, with the following modifications. .. Extracted RNA (8μl) was treated with DNAse, as recommended, then spiked with three reverse transcription/amplification controls (1μl each, containing approximately 10 4 genome copies each of Zika virus (ATCC-VR-1838DQ), murine respirovirus (ATCC-VR-907DQ) and orthoreovirus (ATCC-VR-824DQ) (ATCC Manassas, Virginia, USA). cDNA synthesis was primed randomly using the 3’ terminal N 9 segment of custom oligonucleotide primers (5’-GAT-GAT-AGT-AGG-GCT-TCG-TCA-CNN-NNN-NNN N-3’; Integrated DNA Technologies, Leuven, Belgium), final concentration 5μM. ..

    cDNA Synthesis:

    Article Title: Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens
    Article Snippet: First strand cDNA was synthesized using the Maxima H Minus 1 st Strand cDNA synthesis kit with ds DNase (Thermo Fisher Scientific), in a 20μl reaction volume, according to the manufacturer’s protocol, with the following modifications. .. Extracted RNA (8μl) was treated with DNAse, as recommended, then spiked with three reverse transcription/amplification controls (1μl each, containing approximately 10 4 genome copies each of Zika virus (ATCC-VR-1838DQ), murine respirovirus (ATCC-VR-907DQ) and orthoreovirus (ATCC-VR-824DQ) (ATCC Manassas, Virginia, USA). cDNA synthesis was primed randomly using the 3’ terminal N 9 segment of custom oligonucleotide primers (5’-GAT-GAT-AGT-AGG-GCT-TCG-TCA-CNN-NNN-NNN N-3’; Integrated DNA Technologies, Leuven, Belgium), final concentration 5μM. ..

    Concentration Assay:

    Article Title: Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens
    Article Snippet: First strand cDNA was synthesized using the Maxima H Minus 1 st Strand cDNA synthesis kit with ds DNase (Thermo Fisher Scientific), in a 20μl reaction volume, according to the manufacturer’s protocol, with the following modifications. .. Extracted RNA (8μl) was treated with DNAse, as recommended, then spiked with three reverse transcription/amplification controls (1μl each, containing approximately 10 4 genome copies each of Zika virus (ATCC-VR-1838DQ), murine respirovirus (ATCC-VR-907DQ) and orthoreovirus (ATCC-VR-824DQ) (ATCC Manassas, Virginia, USA). cDNA synthesis was primed randomly using the 3’ terminal N 9 segment of custom oligonucleotide primers (5’-GAT-GAT-AGT-AGG-GCT-TCG-TCA-CNN-NNN-NNN N-3’; Integrated DNA Technologies, Leuven, Belgium), final concentration 5μM. ..



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    murine respirovirus  (ATCC)
    94
    ATCC murine respirovirus
    Red indicates passed and black failed. RNA from murine <t>respirovirus,</t> orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.
    Murine Respirovirus, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+respirovirus/Quantitative+Genomic+RNA+from+Sendai+virus+strain+Cantell/med_rxiv__64898__2026__02__06__26345651-182-29-31
    Average 94 stars, based on 1 article reviews
    murine respirovirus - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Red indicates passed and black failed. RNA from murine respirovirus, orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.

    Journal: medRxiv

    Article Title: Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens

    doi: 10.64898/2026.02.06.26345651

    Figure Lengend Snippet: Red indicates passed and black failed. RNA from murine respirovirus, orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.

    Article Snippet: Extracted RNA (8μl) was treated with DNAse, as recommended, then spiked with three reverse transcription/amplification controls (1μl each, containing approximately 10 4 genome copies each of Zika virus (ATCC-VR-1838DQ), murine respirovirus (ATCC-VR-907DQ) and orthoreovirus (ATCC-VR-824DQ) (ATCC Manassas, Virginia, USA). cDNA synthesis was primed randomly using the 3’ terminal N 9 segment of custom oligonucleotide primers (5’-GAT-GAT-AGT-AGG-GCT-TCG-TCA-CNN-NNN-NNN N-3’; Integrated DNA Technologies, Leuven, Belgium), final concentration 5μM.

    Techniques: Virus, cDNA Synthesis, Extraction, Control, Biomarker Discovery